16.02.2017 Lapatinib resistance in breast cancer cells is accompanied by phosphorylation-mediated reprogramming of glycolysis
We employed explorative mass spectrometry to profile proteome, kinome, and phosphoproteome changes in an established model of lapatinib resistance to systematically investigate initial inhibitor response and subsequent...
The Chair of Proteomics and Bioanalytics contributed three chapters to a new edition of the Methods in Moleulcar Biology series: Proteomics - Methods and Protocols.
We describe ProteomeTools, a project building molecular and digital tools from the human proteome to facilitate biomedical research.
In eukaryotes, the conjugation of the ubiquitinlike protein NEDD8 onto protein targets is an important post-translational modification. Little is known about the role of other deneddylating enzymes the role of neddylation and...
30.10.2016 Phosphoproteome Profiling Reveals Molecular Mechanisms of Growth-Factor-Mediated Kinase Inhibitor Resistance in EGFR-Overexpressing Cancer Cells
Here we show that epidermal growth factor receptor (EGFR) inhibition by Gefitinib is counteracted by growth factors, notably FGF2, and we assessed the global molecular consequences of this resistance at the proteome and...
21.10.2016 Chemical proteomics and structural biology define EPHA2 inhibition by clinical kinase drugs
Here, we used chemical proteomics to survey 235 clinical kinase inhibitors for their kinase selectivity and identified 24 drugs with submicromolar affinities for EPHA2.
Here, we systematically evaluated the concept by screening about 30 compounds with various physico-chemical properties as LC additives.